BIOTREND’s Dual Apoptosis Assay

BIOTREND’s Dual Apoptosis Assay with NucView 488 Caspase-3 Substrate and fluorescent Annexin V

BIOTREND’s apoptose assay kit provides two apoptosis markers, our novel NucView™ 488 caspase-3 substrate and fluorescentAnnexin V, for profiling apoptotic cells and studying the relation of two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation.

Traditionally, caspase activity has been detected either using a membrane-impermeable fluorogenic enzyme substrate such as DEVD-R110, or a fluorescently-labeled inhibitor such as a FLICA reagent. In the former case, cell lysis is required, thus precluding the detection of caspase activity in live cells.

Unlike conventional caspase assays, BIOTREND’s apoptose assay NucView 488 Caspase-3 substrate detects caspase-3 activity within individual whole cells in a non-interfering manner. The substrate consists of a fluorogenic DNA dye and a DEVD substrate moiety specific for caspase-3. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the BIOTREND apoptose assay NucView 488 caspase-3 substrate is bi-functional, allowing detection of caspase-3 activity and visualization of apoptotic nuclear morphology.

BIOTREND’s apoptose assay kit contains reagents sufficient for 50 flow cytometry assays (200 uL assay volume). The number of fluorescence microscopy assays that can be performed with the kit may vary based on the size of culture vessel and staining volume used.

Since 26 years BIOTREND sells worldwide ELISA kits to customers in the biotechnology industry, pharmaceutical and big pharma industry, universities and research clinics as well as Max Planck-, Helmholtz- and Fraunhofer institutes.

BIOTREND runs offices in Cologne - Germany, Wangen/Zurich – Switzerland and Destin – Florida.

For more information about BIOTREND’s Dual Apoptosis Assay elisa please click here...